Saturday, March 30, 2019
Antimicrobial Activity of Coconut Water | Research Proposal
Antimicrobial Activity of cocoanut Water Research ProposalUrinary nerve tract infection (UTI) is the joint term for the heterogenous group of conditions in which there is growth of bacterium in the urinary tract.1 UTIs occur in 3-5% of girls and 1% of boys. later the origin UTI, 60-80% of girls leave behind develop a second UTI within 18 mo. In boys, most UTIs occur during the 1st yr of life. UTIs are much to a greater extent crude in uncircumcised boys. The prevalence of UTIs varies with age. During the 1st yr of life, the male feminine ratio is 2.8-5.4 1. Beyond 1-2 yr, there is a striking female preponderance, with a male female ratio of 1 10.2According to the National ambulatory checkup Care Survey and National Hospital Ambulatory Medical Care Survey, UTI accounted for n earlier 7 billion office visits and 1 million emergency department visits each year.3 UTIs are eventd mainly by colonic bacteria. In females, 75-90% of all infections are caused by Escherichia coli, followed by Klebsiella spp. and genus Proteus spp. more or less series report that in males older than 1 yr of age, Proteus is as common a cause as E. coli.2 UTI can cause significant morbidity if not fitly identified and tempered. Therefore early recognition and prompt treatment is important to prevent late sequelae, such(prenominal) as renal scarring, hypertension, and renal failure.4Coconuts, which are native in our country, spiel an important role in the society. Not only do they proffer shelter and livelihood for mankind but they are also the commencement of important physiologically functional components. Nowadays, cocoa palm has been gaining too much popularity because of its potential difference antimicrobial benefits.5Coconut pissing is incredibly healthy and one of the surmount drinks to hydrate the body. Besides helping to remove toxins from the body and economic aiding digestion, coconuts fetch frightful anti-viral, anti-fungal and anti-microbial proper ties that help to cure the disease.6 It contains mettlesome levels of lauric acid, a substance responsible for these properties.7 up to now, no guinea pig has been done to document or confirm its antimicrobial properties against UTI pathogens, thus, this get was conceptualized.Thus, it is the aim of this study to explore adjunctive treatment for urinary tract infection.REVIEW OF RELATED LITERATURELauric acid which is found in high quantity in Virgin coconut oil was proven to have antibacterial activity against various viruses, protozoal and bacterial pathogens. However, one study conducted here in Davao City dated September 2004 showed that commercially lendable virgin coconut oil has no antibacterial activity against the urinary tract pathogens, E. coli and K. pneumoniae.5SIGNIFICANCE OF THE STUDYUrinary tract infection is a serious health problem affecting millions of people each year. It is treated with various antibacterial drugs which are readily functional in the market. However due to the increasing cost of these drugs, many people cannot afford them and crystalize to self medication with natural remedies. The result of this study leave behind aid the community on victimization an adjunctive medicine that is readily available for the treatment of one of the common diseases in children.OBJECTIVESGeneral ObjectiveTo go steady the antibacterial activity of coconut water use the geographical zone of curtailment on Escherichia coli, Klebsiella pneumoniae and Proteus mirabilis (most common causes of UTI).Specific ObjectivesTo determine the zone of inhibition of coconut water on E. coli, K. pneumoniae and P. mirabilisTo determine which among the bacterial pathogens in UTI is the most sensitive to coconut water.DEFINITION OF TERMS regularize of inhibition this is the clear area formed around the gain vigor publisher dish antenna after 24 hours incubation of the petri dishes.Control disc impregnated disc with Amikacin and Cotrimoxazole used as s tandards for comparing the zone of inhibitions of coconut water against common urinary tract pathogens.Coconut water is the clear liquid privileged young coconutsMETHODOLOGYThe study volition be conducted in a school microbiology laboratory wherein the preparations of the materials and the interpretation of the results will be done.The young coconut fruit will be freshly obtained from the tree and then the water will be separated from its pulp.The test organisms, E. coli, K. pneumoniae and P. mirabilis will be obtained from positive cultures isolated from urine cultures of both pediatric and adult patients. aesthesia testing for each organism will be performed to determine the eccentric of antibiotic that will be used as control for the study.The Mueller-Hinton agar-agar will be used as the medium for the susceptibility testing. The Schieler and Schull filter paper will be used to prepare 6mm disc using a puncher. The materials that will be used in the experiment proper will b e sterilized in the autoclaved at 15psi for 15 minutes. After sterilization, the agar will be dispensed in the sterilized petri dishes with a depth of 5mm and allowed to solidify.Three trials with 9 cultures of each bacterial strain will be tested using the Kirby-Bauer Disc Diffusion Method. Each bacterial strain will be made into a broth happy chance and streaked evenly onto the surface of the medium using a stereotyped cotton swab. The sterile filter paper disc will be dipped into the coconut water. The prepared discs will then be allowed to change for 3-5 minutes after which, they will be on the agar using a sterile forceps and gently pressed down to ensure contact. For the control, commercially impregnated discs with Amikacin and Cotrimoxazole will be used. The plates will be incubated at 37oC for 24 hours and will be investigated for antibacterial activity using the zone of inhibition. The zones of inhibition will be determined in millimeters using a digital caliper. The th rifty zones of inhibition will be classified as follows 0mm-6.99mm as negative, 7mm-12.99mm as weak, 13mm-19.99mm as moderate and 20mm as strong.